A ‘fluffy cloud’ of protein shields water bears’ DNA from radiation, drying and other damage
Source: Science News
Tardigrades may partly owe their ability to survive outer space to having the molecular equivalent of cotton candy.
Water bears, as the creatures are also known, can famously survive just about anything, including being bombarded with X-rays or cosmic rays, or being doused in hydrogen peroxide. Such radiation and chemical exposure result in production of DNA-damaging hydroxyl radicals, molecules composed of oxygen and hydrogen.
Previous research indicated that a protein called Dsup, for damage suppressor, shields the tardigrade species Ramazzottius varieornatus from radiation. When added to human cells, the protein also protects against radiation. Now researchers have found out how.
Dsup surrounds nucleosomes — DNA wound around proteins called histones — “like a fluffy cloud of cotton candy,” molecular biologist James Kadonaga of the University of California, San Diego in La Jolla and colleagues report October 1 in eLife. That cloud keeps hydroxyl radicals away from DNA.
Another tardigrade species Hypsibius exemplaris, previously thought to lack Dsup, has its own version of the protective protein, the researchers discovered. Only about 26 percent of the amino acids in the two species’ Dsup proteins are alike, but both shroud DNA against damage.
Kadonaga says the proteins probably evolved to protect tardigrades from hydroxy radicals when the moss-dwellers are dried out, a frequent occurrence. Drying increases the concentration of DNA-dinging radicals in cells. And damage can’t be repaired while the animals are dormant in their desiccated state. Since X-rays also form hydroxy radicals, tardigrades “just happen to be X-ray resistant,” too, he says.
Humans have similar proteins called high mobility group nucleosome-binding proteins or HMGNs. But the researchers don’t yet know whether the human proteins also form a similar shield against DNA-damaging chemicals.